Streamline coliforms & E. coli testing with Policontrol

A complete MPN workflow for microbiological water testing labs - ColiControl (ONPG–MUG) + PoliTray MPN (97 wells) + Poli Sealer + MPN Reader.

Advanced 97 Well MPN Platform for Rapid Microbiological Water Analysis.
In partnership with:
Policontrol

Product Overview

ColiControl is a culture medium for the simultaneous detection of total coliforms and Escherichia coli in water samples, used within the PoliTray MPN workflow. Using ONPG and MUG technology, it delivers reliable results within 24 hours.

It offers simple preparation, high sensitivity, and neutralises chlorine interference to ensure accurate results. Validated to ISO 17025 and compliant with Standard Methods, ColiControl provides a dependable solution for routine microbiological water testing.

Applications:

1)   Drinking Water

      • Bottled Water
      • Still Water
      • Sparkling and carbonated water (Presence or Absence testing with ColiControl 100)

2)   Environmental Waters

      • Fresh Water
      • Well Water
      • Surface Water
      • Ground Water
      • Marine Water
      • Wastewater

Products in the Workflow

ColiControl (ONPG MUG Test Kit)

What It Is
ColiControl is a selective and differential, ready-to-use dehydrated medium for the simultaneous detection of total coliforms and Escherichia coli in water using ONPG and MUG enzymatic substrate technology. The medium is added directly to the sample, simplifying preparation and supporting consistent, reliable results.

How It Is Used
Suitable for both Presence or Absence screening and quantitative enumeration using the MPN method. It integrates directly with the PoliTray system to support complete microbiological water testing workflows.

Key Manufacturer Specifications

Limit of Detection: 1 CFU in a 100 mL sample

Incubation Time: 16 to 48 hours

Storage: 2 to 30 degrees Celsius, 4 to 7 degrees Celsius recommended

Pack Size: 100 tests

Optional: Add-on inhibitors are available to reduce interference from non-target organisms

PoliTray MPN (97 well tray)

What It Is
PoliTray is a sterile 97-well MPN tray designed for the quantitative detection of total coliforms and Escherichia coli in water samples. Used together with ColiControl medium, it enables accurate enumeration based on the Most Probable Number method.

How It Is Used
After the sample and reagent are combined, the mixture is dispensed into the tray and sealed using the Poli Sealer. Following incubation, positive wells are counted manually or using the MPN Reader to determine the bacterial concentration.

Method Reference
MPN method according to Standard Methods for the Examination of Water and Wastewater.

Key Manufacturer Specifications

Wells: 97

Sample volume: 100 mL

Quantification range: Up to 2419.2 MPN of total coliforms and Escherichia coli

Quality assurance: Supplied sterile with sterilisation report, sealing integrity verification, and quality control certification

MPN Reader (automated tray reading + MPN calculation)

What It Is
The MPN Reader is an automated system designed to read incubated PoliTray MPN trays and calculate results quickly and accurately. It captures high-resolution images under UV and white light to detect positive wells and determine the Most Probable Number for total coliforms and Escherichia coli.

How It Is Used
After incubation, the sealed tray is placed into the reader, where the system automatically analyses the results, calculates the MPN value, and stores the data and images for reporting, traceability, and record keeping.

Key Manufacturer Specification

Reading time: Approximately 7 seconds per tray

Data handling: Stores results and tray images for documentation and quality control

Poli Sealer (MPN tray sealer)

What It Is
The Poli Sealer is a motor-driven heated roller sealer designed to securely seal filled MPN trays before incubation. It ensures proper sample containment, prevents leakage, and supports accurate enumeration results.

How It Is Used
After the sample and reagent are dispensed into the PoliTray, the tray is passed through the sealer, which applies consistent heat and pressure to seal each well. This prepares the tray for safe handling and incubation.

Key Manufacturer Specifications

Sealing mechanism: Motor-driven heated roller

Controls: Selectable temperature with reversion button

Pre-heating time: Ready for use in under 5 minutes

Compatibility: Suitable for PoliTray and other compatible MPN tray brands

Typical Workflow

Prepare 100 mL sample and add ColiControl → Dispense into PoliTray → Seal using Poli Sealer → Incubate 16–48 hours

→ Read and report results

Key Benefits

  • Standardised 97-well MPN workflow for consistent results
  • Automated tray reading and MPN calculation in approximately 7 seconds per tray
  • Automatic image and data storage for traceability and reporting
  • Suitable for routine drinking water, environmental, and wastewater testing
Available in South Africa