The World Health Organization (WHO) has published global cholera statistics for 2023, showing an increase of 13% in reported cases and 71% in deaths compared to 2022. Over 4000 people died last year from this preventable and treatable disease. This report shows that cholera remains a public health problem, especially regarding water sanitation and hygiene.
What is Cholera?
Cholera is an acute diarrheal disease that is spread by contaminated food or water by Vibrio cholerae (V. cholerae) more frequently seen in Africa and Southeast Asia. Cholera needs to be treated immediately upon the start of the symptoms, or else it can develop into severe dehydration and even death within hours. The start of the symptoms is usually 12 hours to 5 days after the infection.
Cholera is closely linked to inadequate access to clean water and lack of sanitation. Humanitarian crises that cause displacement of populations into crowded areas, climate change, and unplanned urbanization increase the risk of cholera transmission.
Diagnosis of cholera is performed by laboratory tests like culture or Real-Time PCR from stool samples. Rapid access to treatment is essential during cholera outbreaks, since with early and proper treatment, the case fatality rate remains below 1%. However, diagnosis can be tricky since the cholera symptoms (diarrhea, nausea, vomiting, and dehydration) can also be present in other gastrointestinal infections. Real-time PCR Kits like Bosphore Gastroenteritis Panel Kit v4 can detect 24 gastrointestinal pathogens including V. cholerae therefore enabling prompt start of treatment.
Bosphore Gastroenteritis Panel Kit v4 by Anatolia Geneworks is an effective test for Cholera
Gastrointestinal tract infections (gastroenteritis) include bacterial, viral and protozoan pathogens that cause inflammation of the mucous membranes of the gastrointestinal tract. They rank as the second highest rates of mortality and morbidity following pneumonia, especially in children under 5 years of age in developing countries. Apart from the serious consequences of gastroenteritis infections, the disease is a burden on the health services in developed countries. Therefore, rapid and accurate diagnosis is very important worldwide.
Features
Single Tube Master Mix
Internal Control for Each Reaction
Compatibility with Various Systems
Package Size Options
Expert Support
Workflow
Bosphore Gastroenteritis Panel Kit v4 detects Sapovirus (I, II, IV, V), Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, Adenovirus, Clostridium difficile toxin A/B, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli VTEC (E. coli STEC (STX1/STX2)), Escherichia coli EIEC/Shigella, Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., Giardia lamblia, Escherichia coli EPEC, Escherichia coli ETEC lt/st, Cyclospora cayetanensis, Escherichia coli EAEC, Escherichia coli STEC O157:H7, Plesiomonas shigelloides, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus from stool samples.
Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.
| FAM | HEX | Cy5 | Texas RED | |
| Tube 1 | Sapovirus (I, II, IV, V) | Astrovirus | Rotavirus A | Internal Control |
| Tube 2 | Norovirus GI | Norovirus GII | Adenovirus | Internal Control |
| Tube 3 | Clostridium difficile toxin A/B | Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari) |
Salmonella spp. | Internal Control |
| Tube 4 | E. coli (VTEC) (E. coli STEC (STX1/STX2)) | E. coli (EIEC)/ Shigella spp. | Yersinia enterocolitica | Internal Control |
| Tube 5 | Entamoeba histolytica | Cryptosporidium spp. | Giardia lamblia (Giardia duodenalis) | Internal Control |
| Tube 6 | E. coli (EPEC) | E. coli (ETEC lt/st) | Cyclospora cayetanensis | Internal Control |
| Tube 7 | E. coli (EAEC) | E. coli (STEC O157:H7) | Plesiomonas shigelloides | Internal Control |
| Tube 8 | Vibrio cholerae | Vibrio parahaemolyticus | Vibrio vulnificus | Internal Control |
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, and application mistakes. The internal control can be added either during DNA extraction or PCR step. The kit also includes positive controls.